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inactivated hff1  (ATCC)


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    Structured Review

    ATCC inactivated hff1
    The feeder cell layer promotes DNA replication in neoblasts (A) Schematic diagram and representative images showing SiRNeoblasts seeded on <t>HFF1</t> and MEF feeder layer, respectively. Scale bars, 50 μm. (B) Cell morphology of SiRNeoblasts cultured on HFF1 or MEF feeder layer for 1, 3, and 7 days. Scale bars, 50 μm. (C) Relative F-ara-EdU + cell ratio of SiRNeoblasts cultured in the plastic flat plate, HFF1, and MEF feeder layer conditions for 3 days. Three wells were assayed in each group. Data are represented as mean ± SEM. Binomial GLM calculates p values. ∗∗∗∗ p < 0.0001. The ratio was calculated as (total number of F-ara-EdU + cells)/(initial number of seeded cells). (D) Heatmap shows expression of genes related to nucleoside metabolism in cells without culture or cells cultured in the plastic flat plate, HFF1, and MEF feeder layer conditions for 1 day. Each group contains three replicates. See also , , and .
    Inactivated Hff1, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1501 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/inactivated hff1/product/ATCC
    Average 99 stars, based on 1501 article reviews
    inactivated hff1 - by Bioz Stars, 2026-05
    99/100 stars

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    1) Product Images from "Enhanced cell contact and planarian tissue extract increase DNA replication and viability for neoblast cultivation"

    Article Title: Enhanced cell contact and planarian tissue extract increase DNA replication and viability for neoblast cultivation

    Journal: iScience

    doi: 10.1016/j.isci.2026.115274

    The feeder cell layer promotes DNA replication in neoblasts (A) Schematic diagram and representative images showing SiRNeoblasts seeded on HFF1 and MEF feeder layer, respectively. Scale bars, 50 μm. (B) Cell morphology of SiRNeoblasts cultured on HFF1 or MEF feeder layer for 1, 3, and 7 days. Scale bars, 50 μm. (C) Relative F-ara-EdU + cell ratio of SiRNeoblasts cultured in the plastic flat plate, HFF1, and MEF feeder layer conditions for 3 days. Three wells were assayed in each group. Data are represented as mean ± SEM. Binomial GLM calculates p values. ∗∗∗∗ p < 0.0001. The ratio was calculated as (total number of F-ara-EdU + cells)/(initial number of seeded cells). (D) Heatmap shows expression of genes related to nucleoside metabolism in cells without culture or cells cultured in the plastic flat plate, HFF1, and MEF feeder layer conditions for 1 day. Each group contains three replicates. See also , , and .
    Figure Legend Snippet: The feeder cell layer promotes DNA replication in neoblasts (A) Schematic diagram and representative images showing SiRNeoblasts seeded on HFF1 and MEF feeder layer, respectively. Scale bars, 50 μm. (B) Cell morphology of SiRNeoblasts cultured on HFF1 or MEF feeder layer for 1, 3, and 7 days. Scale bars, 50 μm. (C) Relative F-ara-EdU + cell ratio of SiRNeoblasts cultured in the plastic flat plate, HFF1, and MEF feeder layer conditions for 3 days. Three wells were assayed in each group. Data are represented as mean ± SEM. Binomial GLM calculates p values. ∗∗∗∗ p < 0.0001. The ratio was calculated as (total number of F-ara-EdU + cells)/(initial number of seeded cells). (D) Heatmap shows expression of genes related to nucleoside metabolism in cells without culture or cells cultured in the plastic flat plate, HFF1, and MEF feeder layer conditions for 1 day. Each group contains three replicates. See also , , and .

    Techniques Used: Cell Culture, Expressing

    Planarian extract improves cell viability (A and B) Relative PI + dead cell ratio of SiRNeoblasts cultured in the plastic flat plate, the U-bottom plate, HFF1, and MEF feeder layer conditions for 3 days (A) and 7 days (B), respectively. Three wells were assayed in each group. Data are represented as mean ± SEM. All other experimental groups were compared to the plastic flat plate group to assess statistical significance. One-way ANOVA with the Tukey test calculates adjusted p values. ∗∗0.001 < p < 0.01, ∗∗∗∗ p < 0.0001. n.s., no significance. (C) Workflow of preparing planarian extract. (D) Relative PI + dead cell ratio of SiRNeoblasts cultured for 1 and 3 days under five conditions. These five conditions include the plastic flat plate with or without planarian extract, the U-bottom plate with or without planarian extract, and HFF1 feeder layer. Three wells were assayed in each group. Data are represented as mean ± SEM. One-way ANOVA with the Tukey test calculates adjusted p values. ∗0.01 < p < 0.05, ∗∗0.001 < p < 0.01, ∗∗∗0.0001 < p < 0.001. n.s., no significance. (E) Relative F-ara-EdU + cell ratio of SiRNeoblasts cultured for 1 day under five conditions. Three wells were assayed in each group. Data are represented as mean ± SEM. Binomial GLM calculates p values. ∗∗∗∗ p < 0.0001. The ratio was calculated as (total number of F-ara-EdU + cells)/(initial number of seeded cells). (F) Relative piwi-1 + cell ratio of SiRNeoblasts cultured for 7 days under six conditions. The differences among these six conditions lie in three factors: aggregate formation, the addition of extract or not, and the duration of extract exposure. Three wells were assayed in each group. Data are represented as mean ± SEM. One-way ANOVA with the Tukey test calculates adjusted p values. ∗0.01 < p < 0.05, ∗∗0.001 < p < 0.01, n.s., no significance. The ratio was calculated as (total number of piwi-1 + cells)/(initial number of seeded cells). (G) Relative dead cell ratio of SiRNeoblasts cultured for 7 days under six conditions. Three wells were assayed in each group. Data are represented as mean ± SEM. One-way ANOVA with the Tukey test calculates adjusted p values. ∗0.01 < p < 0.05, ∗∗∗0.0001 < p < 0.001, ∗∗∗∗ p < 0.0001. See also .
    Figure Legend Snippet: Planarian extract improves cell viability (A and B) Relative PI + dead cell ratio of SiRNeoblasts cultured in the plastic flat plate, the U-bottom plate, HFF1, and MEF feeder layer conditions for 3 days (A) and 7 days (B), respectively. Three wells were assayed in each group. Data are represented as mean ± SEM. All other experimental groups were compared to the plastic flat plate group to assess statistical significance. One-way ANOVA with the Tukey test calculates adjusted p values. ∗∗0.001 < p < 0.01, ∗∗∗∗ p < 0.0001. n.s., no significance. (C) Workflow of preparing planarian extract. (D) Relative PI + dead cell ratio of SiRNeoblasts cultured for 1 and 3 days under five conditions. These five conditions include the plastic flat plate with or without planarian extract, the U-bottom plate with or without planarian extract, and HFF1 feeder layer. Three wells were assayed in each group. Data are represented as mean ± SEM. One-way ANOVA with the Tukey test calculates adjusted p values. ∗0.01 < p < 0.05, ∗∗0.001 < p < 0.01, ∗∗∗0.0001 < p < 0.001. n.s., no significance. (E) Relative F-ara-EdU + cell ratio of SiRNeoblasts cultured for 1 day under five conditions. Three wells were assayed in each group. Data are represented as mean ± SEM. Binomial GLM calculates p values. ∗∗∗∗ p < 0.0001. The ratio was calculated as (total number of F-ara-EdU + cells)/(initial number of seeded cells). (F) Relative piwi-1 + cell ratio of SiRNeoblasts cultured for 7 days under six conditions. The differences among these six conditions lie in three factors: aggregate formation, the addition of extract or not, and the duration of extract exposure. Three wells were assayed in each group. Data are represented as mean ± SEM. One-way ANOVA with the Tukey test calculates adjusted p values. ∗0.01 < p < 0.05, ∗∗0.001 < p < 0.01, n.s., no significance. The ratio was calculated as (total number of piwi-1 + cells)/(initial number of seeded cells). (G) Relative dead cell ratio of SiRNeoblasts cultured for 7 days under six conditions. Three wells were assayed in each group. Data are represented as mean ± SEM. One-way ANOVA with the Tukey test calculates adjusted p values. ∗0.01 < p < 0.05, ∗∗∗0.0001 < p < 0.001, ∗∗∗∗ p < 0.0001. See also .

    Techniques Used: Cell Culture



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    inactivated hff1  (ATCC)
    99
    ATCC inactivated hff1
    The feeder cell layer promotes DNA replication in neoblasts (A) Schematic diagram and representative images showing SiRNeoblasts seeded on <t>HFF1</t> and MEF feeder layer, respectively. Scale bars, 50 μm. (B) Cell morphology of SiRNeoblasts cultured on HFF1 or MEF feeder layer for 1, 3, and 7 days. Scale bars, 50 μm. (C) Relative F-ara-EdU + cell ratio of SiRNeoblasts cultured in the plastic flat plate, HFF1, and MEF feeder layer conditions for 3 days. Three wells were assayed in each group. Data are represented as mean ± SEM. Binomial GLM calculates p values. ∗∗∗∗ p < 0.0001. The ratio was calculated as (total number of F-ara-EdU + cells)/(initial number of seeded cells). (D) Heatmap shows expression of genes related to nucleoside metabolism in cells without culture or cells cultured in the plastic flat plate, HFF1, and MEF feeder layer conditions for 1 day. Each group contains three replicates. See also , , and .
    Inactivated Hff1, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/inactivated hff1/product/ATCC
    Average 99 stars, based on 1 article reviews
    inactivated hff1 - by Bioz Stars, 2026-05
    99/100 stars
    		  Buy from Supplier

    Image Search Results


    The feeder cell layer promotes DNA replication in neoblasts (A) Schematic diagram and representative images showing SiRNeoblasts seeded on HFF1 and MEF feeder layer, respectively. Scale bars, 50 μm. (B) Cell morphology of SiRNeoblasts cultured on HFF1 or MEF feeder layer for 1, 3, and 7 days. Scale bars, 50 μm. (C) Relative F-ara-EdU + cell ratio of SiRNeoblasts cultured in the plastic flat plate, HFF1, and MEF feeder layer conditions for 3 days. Three wells were assayed in each group. Data are represented as mean ± SEM. Binomial GLM calculates p values. ∗∗∗∗ p < 0.0001. The ratio was calculated as (total number of F-ara-EdU + cells)/(initial number of seeded cells). (D) Heatmap shows expression of genes related to nucleoside metabolism in cells without culture or cells cultured in the plastic flat plate, HFF1, and MEF feeder layer conditions for 1 day. Each group contains three replicates. See also , , and .

    Journal: iScience

    Article Title: Enhanced cell contact and planarian tissue extract increase DNA replication and viability for neoblast cultivation

    doi: 10.1016/j.isci.2026.115274

    Figure Lengend Snippet: The feeder cell layer promotes DNA replication in neoblasts (A) Schematic diagram and representative images showing SiRNeoblasts seeded on HFF1 and MEF feeder layer, respectively. Scale bars, 50 μm. (B) Cell morphology of SiRNeoblasts cultured on HFF1 or MEF feeder layer for 1, 3, and 7 days. Scale bars, 50 μm. (C) Relative F-ara-EdU + cell ratio of SiRNeoblasts cultured in the plastic flat plate, HFF1, and MEF feeder layer conditions for 3 days. Three wells were assayed in each group. Data are represented as mean ± SEM. Binomial GLM calculates p values. ∗∗∗∗ p < 0.0001. The ratio was calculated as (total number of F-ara-EdU + cells)/(initial number of seeded cells). (D) Heatmap shows expression of genes related to nucleoside metabolism in cells without culture or cells cultured in the plastic flat plate, HFF1, and MEF feeder layer conditions for 1 day. Each group contains three replicates. See also , , and .

    Article Snippet: Mitotically inactivated HFF1 (ATCC, SCRC-1041) and MEF (embryonic day 13.5) cells treated with 10 μg/mL Mitomycin C (Sigma, M5353) for 3 hours were recovered one day prior to seeding planarian cells.

    Techniques: Cell Culture, Expressing

    Planarian extract improves cell viability (A and B) Relative PI + dead cell ratio of SiRNeoblasts cultured in the plastic flat plate, the U-bottom plate, HFF1, and MEF feeder layer conditions for 3 days (A) and 7 days (B), respectively. Three wells were assayed in each group. Data are represented as mean ± SEM. All other experimental groups were compared to the plastic flat plate group to assess statistical significance. One-way ANOVA with the Tukey test calculates adjusted p values. ∗∗0.001 < p < 0.01, ∗∗∗∗ p < 0.0001. n.s., no significance. (C) Workflow of preparing planarian extract. (D) Relative PI + dead cell ratio of SiRNeoblasts cultured for 1 and 3 days under five conditions. These five conditions include the plastic flat plate with or without planarian extract, the U-bottom plate with or without planarian extract, and HFF1 feeder layer. Three wells were assayed in each group. Data are represented as mean ± SEM. One-way ANOVA with the Tukey test calculates adjusted p values. ∗0.01 < p < 0.05, ∗∗0.001 < p < 0.01, ∗∗∗0.0001 < p < 0.001. n.s., no significance. (E) Relative F-ara-EdU + cell ratio of SiRNeoblasts cultured for 1 day under five conditions. Three wells were assayed in each group. Data are represented as mean ± SEM. Binomial GLM calculates p values. ∗∗∗∗ p < 0.0001. The ratio was calculated as (total number of F-ara-EdU + cells)/(initial number of seeded cells). (F) Relative piwi-1 + cell ratio of SiRNeoblasts cultured for 7 days under six conditions. The differences among these six conditions lie in three factors: aggregate formation, the addition of extract or not, and the duration of extract exposure. Three wells were assayed in each group. Data are represented as mean ± SEM. One-way ANOVA with the Tukey test calculates adjusted p values. ∗0.01 < p < 0.05, ∗∗0.001 < p < 0.01, n.s., no significance. The ratio was calculated as (total number of piwi-1 + cells)/(initial number of seeded cells). (G) Relative dead cell ratio of SiRNeoblasts cultured for 7 days under six conditions. Three wells were assayed in each group. Data are represented as mean ± SEM. One-way ANOVA with the Tukey test calculates adjusted p values. ∗0.01 < p < 0.05, ∗∗∗0.0001 < p < 0.001, ∗∗∗∗ p < 0.0001. See also .

    Journal: iScience

    Article Title: Enhanced cell contact and planarian tissue extract increase DNA replication and viability for neoblast cultivation

    doi: 10.1016/j.isci.2026.115274

    Figure Lengend Snippet: Planarian extract improves cell viability (A and B) Relative PI + dead cell ratio of SiRNeoblasts cultured in the plastic flat plate, the U-bottom plate, HFF1, and MEF feeder layer conditions for 3 days (A) and 7 days (B), respectively. Three wells were assayed in each group. Data are represented as mean ± SEM. All other experimental groups were compared to the plastic flat plate group to assess statistical significance. One-way ANOVA with the Tukey test calculates adjusted p values. ∗∗0.001 < p < 0.01, ∗∗∗∗ p < 0.0001. n.s., no significance. (C) Workflow of preparing planarian extract. (D) Relative PI + dead cell ratio of SiRNeoblasts cultured for 1 and 3 days under five conditions. These five conditions include the plastic flat plate with or without planarian extract, the U-bottom plate with or without planarian extract, and HFF1 feeder layer. Three wells were assayed in each group. Data are represented as mean ± SEM. One-way ANOVA with the Tukey test calculates adjusted p values. ∗0.01 < p < 0.05, ∗∗0.001 < p < 0.01, ∗∗∗0.0001 < p < 0.001. n.s., no significance. (E) Relative F-ara-EdU + cell ratio of SiRNeoblasts cultured for 1 day under five conditions. Three wells were assayed in each group. Data are represented as mean ± SEM. Binomial GLM calculates p values. ∗∗∗∗ p < 0.0001. The ratio was calculated as (total number of F-ara-EdU + cells)/(initial number of seeded cells). (F) Relative piwi-1 + cell ratio of SiRNeoblasts cultured for 7 days under six conditions. The differences among these six conditions lie in three factors: aggregate formation, the addition of extract or not, and the duration of extract exposure. Three wells were assayed in each group. Data are represented as mean ± SEM. One-way ANOVA with the Tukey test calculates adjusted p values. ∗0.01 < p < 0.05, ∗∗0.001 < p < 0.01, n.s., no significance. The ratio was calculated as (total number of piwi-1 + cells)/(initial number of seeded cells). (G) Relative dead cell ratio of SiRNeoblasts cultured for 7 days under six conditions. Three wells were assayed in each group. Data are represented as mean ± SEM. One-way ANOVA with the Tukey test calculates adjusted p values. ∗0.01 < p < 0.05, ∗∗∗0.0001 < p < 0.001, ∗∗∗∗ p < 0.0001. See also .

    Article Snippet: Mitotically inactivated HFF1 (ATCC, SCRC-1041) and MEF (embryonic day 13.5) cells treated with 10 μg/mL Mitomycin C (Sigma, M5353) for 3 hours were recovered one day prior to seeding planarian cells.

    Techniques: Cell Culture